How to easily participate in cancer research?
The Institut Bordet Tumour Bank or Tumour Biobank is a collection of samples of human body material (tumour tissue residue, blood samples) and of associated data preserved in optimum conditions for their future use in research. It constitutes a crucial link between diagnosis and fundamental, translational and clinical research. Its activity is subject to strict legislation.
The development of personalised medicine and of immunotherapy in oncology requires the exploration of new biomarkers identifiable in tumour tissue, but also in blood. The role of the Tumour Bank is to collect and supply to scientific research laboratories very high quality, diversified, biological samples in sufficient quantity, accompanied by complete clinical data to enable them to carry out their research. The objective is to understand how tumour ‘machinery’ operates and the strategy that allows it to escape the vigilance and immune defences of its host.
The Tumour Bank forms part of the Anatomical Pathology Department, enabling full and accurate analysis of tumours by pathologists prior to their preservation. It also forms an integral part of other Belgian biobanks – the Cancer Register Virtual Catalogue, BWB (Wallonia-Brussels BioBank) and of 2 European biobanks: the BBMRI and ESBB. Contact via these networks between different Belgian and international research teams facilitates the establishment of links and skill sharing.
The Institut Bordet Tumour Bank has been ISO90001 certified since 2012 – a guarantee of quality. The robust system put in place for the quality control of our samples enables us to support numerous research projects every year. This is evidenced in the quality of the scientific publications that result from these.
- Created: 2010
- More than 16,000 frozen tissue samples available
- Samples provided by more than 10,000 patients
- More than 65 projects using the samples collected
- Digital Pathology
Digital pathology is becoming increasingly important in current practice, particularly in research. The Friends of the Institut Bordet have funded part of our Digital Pathology platform. The process of digitising microscopy slides makes it possible to use software to process images. As a result, precise analysis of different cell populations – solid tumour inflammatory infiltrates, for example – can be carried out. These studies will be transposed to a clinical setting, notably to assess whether or not a particular immunotherapy treatment might be beneficial to patients. In addition, hot-spots (concentrations of cell markers) are identified and analysed.
- Laser Microdissection
Laser microdissection or Laser Capture/Cutting Microdissection (LCM) is a technology that allows the isolation, under morphological control, of cells of interest from tissue sections using a laser beam. This project, supported by the Friends of the Institut Bordet, will make it possible to study tumour heterogeneity at single-cell-type level.
The Tumour Bank not only supplies biological material, it also develops its own scientific projects.
Quality control via infrared spectroscopy.
Analysis of the quality and preservation (stability) of tumour bank frozen sample proteins via infrared spectroscopy
- Project holders : Ligia Craciun
- Collaboration : ULB, BBMRI
Tumour bank extension to blood/plasma samples thanks to the funding of plasma stabilising tubes by the Friends of the Institut Bordet. Qualitative and quantitative analysis of tumour DNA in plasma.
Analysis of intra- and peri-tumoural inflammatory infiltrate in patients who have undergone surgery for hepatocellular carcinoma after intra-arterial radioembolisation
New global analysis of the microRNA transcriptome of primary tumors and lymph node metastases of papillary thyroid cancer.
Saiselet M, Gacquer D, Spinette A, Craciun L, Decaussin-Petrucci M, Andry G, Detours V, Maenhaut C.
BMC Genomics. 2015 Oct 21;16:828.
Evaluation of quantitative polymerase chain reaction markers for the detection of breast cancer cells in ovarian tissue stored for fertility preservation.
Bockstaele L, Boulenouar S, Van Den Steen G, Dechène J, Tsepelidis S, Craciun L, Noël JC, Demeestere I.
Fertil Steril. 2015 Aug;104(2):410-7.e4.
Tumor-infiltrating lymphocyte composition, organization and PD-1/ PD-L1 expression are linked in breast cancer.
Buisseret L, Garaud S, de Wind A, Van den Eynden G, Boisson A, Solinas C, Gu-Trantien C, Naveaux C, Lodewyckx JN, Duvillier H, Craciun L, Veys I, Larsimont D, Piccart-Gebhart M, Stagg J, Sotiriou C, Willard-Gallo K .
Oncoimmunology. 2016 Dec 14;6(1)
Inflammatory Stroma of Lymphoepithelioma-like Carcinoma of the Cervix: Immunohistochemical Study of 3 Cases and Review of the Literature.
Philippe A, Rassy M, Craciun L, Naveaux C, Willard-Gallo K, Larsimont D, Veys I.
Int J Gynecol Pathol. 2017 Aug 1.
CDK4 phosphorylation status and a linked gene expression profile predict sensitivity to palbociclib.
Raspé E, Coulonval K, Pita JM, Paternot S, Rothé F, Twyffels L, Brohée S, Craciun L, Larsimont D, Kruys V, Sandras F, Salmon I, Van Laere S, Piccart M, Ignatiadis M, Sotiriou C, Roger PP.
EMBO Mol Med. 2017 Aug;9(8):1052-1066.
Thyroid follicular adenomas and carcinomas: molecular profiling provides evidence for a continuous evolution.
Dom G, Frank S, Floor S, Kehagias P, Libert F, Hoang C, Andry G , Craciun L, Spinette A, de Saint Aubin N, Tresallet C, Tissier F, Savagner F, Majjaj S, Gutierrez-Roelens I, Marbaix E, Dumont J, Maenhaut C.
Oncotarget nov 2017
Selective internal radiation therapy (SIRT) promotes the recruitment of tumor-infiltrating lymphocytes and enhances cytotoxic activity in hepatocellular carcinoma.
Craciun L, de Wind R, Demetter P, Lucidi V, Michiels S, Garaud S, Naveaux C, Gomez Galdon M, Hendlisz A, Willard Gallo K, Flamen P, Larsimont D, Donckier V.
Abstract ESMO Immuno Oncology congress, dec 2017